Premium
Scaling Up DNA Origami Lattice Assembly
Author(s) -
Xin Yang,
Shen Boxuan,
Kostiainen Mauri A.,
Grundmeier Guido,
Castro Mario,
Linko Veikko,
Keller Adrian
Publication year - 2021
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202100784
Subject(s) - dna origami , nanoscopic scale , materials science , mica , nanotechnology , lattice (music) , homogeneous , fabrication , nanostructure , scaling , nanolithography , atomic force microscopy , geometry , physics , mathematics , statistical physics , alternative medicine , medicine , pathology , acoustics , composite material
Abstract The surface‐assisted hierarchical assembly of DNA origami nanostructures is a promising route to fabricate regular nanoscale lattices. In this work, the scalability of this approach is explored and the formation of a homogeneous polycrystalline DNA origami lattice at the mica‐electrolyte interface over a total surface area of 18.75 cm 2 is demonstrated. The topological analysis of more than 50 individual AFM images recorded at random locations over the sample surface showed only minuscule and random variations in the quality and order of the assembled lattice. The analysis of more than 450 fluorescence microscopy images of a quantum dot‐decorated DNA origami lattice further revealed a very homogeneous surface coverage over cm 2 areas with only minor boundary effects at the substrate edges. At total DNA costs of € 0.12 per cm 2 , this large‐scale nanopatterning technique holds great promise for the fabrication of functional surfaces.