Premium
Click and Release Chemistry for Activity‐Based Purification of β‐Lactam Targets
Author(s) -
Saidjalolov Saidbakhrom,
Braud Emmanuelle,
Edoo Zainab,
Iannazzo Laura,
Rusconi Filippo,
Riomet Margaux,
Sallustrau Antoine,
Taran Frédéric,
Arthur Michel,
Fonvielle Matthieu,
EtheveQuelquejeu Mélanie
Publication year - 2021
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202100653
Subject(s) - bioorthogonal chemistry , click chemistry , chemistry , combinatorial chemistry , alkyne , linker , moiety , peptidoglycan , acylation , cycloaddition , enzyme , biochemistry , stereochemistry , catalysis , computer science , operating system
β‐Lactams, the cornerstone of antibiotherapy, inhibit multiple and partially redundant targets referred to as transpeptidases or penicillin‐binding proteins. These enzymes catalyze the essential cross‐linking step of the polymerization of cell wall peptidoglycan. The understanding of the mechanisms of action of β‐lactams and of resistance to these drugs requires the development of reliable methods to characterize their targets. Here, we describe an activity‐based purification method of β‐lactam targets based on click and release chemistry. We synthesized alkyne‐carbapenems with suitable properties with respect to the kinetics of acylation of a model target, the Ldt fm L,D‐transpeptidase, the stability of the resulting acylenzyme, and the reactivity of the alkyne for the cycloaddition of an azido probe containing a biotin moiety for affinity purification and a bioorthogonal cleavable linker. The probe provided access to the fluorescent target in a single click and release step.