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STED Imaging the Dynamics of Lysosomes by Dually Fluorogenic Si‐Rhodamine
Author(s) -
Fan Mengting,
An Haiyan,
Wang Chuanfeng,
Huo Shuhui,
Wang Ting,
Cui Xiaoyan,
Zhang Dazhi
Publication year - 2021
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202100623
Subject(s) - sted microscopy , fluorescence , rhodamine , biophysics , chemistry , microscopy , fluorescence lifetime imaging microscopy , fluorescence microscope , organelle , fluorophore , live cell imaging , rhodamine b , stimulated emission , biochemistry , biology , optics , cell , physics , laser , photocatalysis , catalysis
Super‐resolution microscopy (SRM) imaging of the finite subcellular structures and subtle bioactivities inside organelles delivers abundant cellular information with high fidelity to unravel the intricate biological processes. An ideal fluorescent probe with precise control of fluorescence is critical in SRM technique like stimulated emission depletion (STED). Si‐rhodamine was decorated with both targeting group and H + ‐receptor, affording the dually fluorogenic Si‐rhodamine in which the NIR fluorescence was efficiently controlled by the coalescent of spirolactone‐zwitterion equilibrium and PeT mechanism. The dually fluorogenic characters of the probe offer a perfect mutual enhancement in sensitivity, specificity and spatial resolution. Strong fluorescence only released in the existence of targeting protein at acidic lysosomal pH, ensured precisely tracking the dynamic of lysosomal structure and pH in living cells by STED.