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Extended Peptide Nucleic Acid Nucleobases Based on Isoorotic Acid for the Recognition of A–U Base Pairs in Double‐Stranded RNA
Author(s) -
Brodyagin Nikita,
Maryniak Aubrey L.,
Kumpina Ilze,
Talbott John M.,
Katkevics Martins,
Rozners Eriks,
MacKay James A.
Publication year - 2021
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202005401
Subject(s) - nucleobase , isothermal titration calorimetry , peptide nucleic acid , nucleic acid , rna , chemistry , base pair , nucleic acid analogue , peptide , dna , biochemistry , stereochemistry , combinatorial chemistry , nucleic acid thermodynamics , gene
Peptide nucleic acids (PNA) with extended isoorotamide containing nucleobases ( Io ) were designed for binding A–U base pairs in double‐stranded RNA. Isothermal titration calorimetry and UV thermal melting experiments revealed improved affinity for A–U using the Io scaffold in PNA. PNAs having four sequential Io extended nucleobases maintained high binding affinity.