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Cyclo‐Ketal Xanthene Dyes: A New Class of Near‐Infrared Fluorophores for Super‐Resolution Imaging of Live Cells
Author(s) -
Zhang Xinfu,
Chen Lingcheng,
Huang Zhenlong,
Ling Ni,
Xiao Yi
Publication year - 2021
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202005296
Subject(s) - fluorescence , fluorophore , xanthene , resolution (logic) , chemistry , fluorescence lifetime imaging microscopy , near infrared spectroscopy , biophysics , optics , photochemistry , computer science , physics , biology , artificial intelligence
Newly emerging super‐resolution imaging techniques provide opportunities for precise observations on cellular microstructures. However, they also impose severe demands on fluorophores. Here, we develop a new series of NIR xanthene dyes, named as KRh s, by replacing the 10‐position O of rhodamines with a cyclo‐ketal. KRh s display an intense NIR emission peak at 700 nm with fluorescence quantum yields up to 0.64. More importantly, they, without the aid of enhancing buffer, exhibit stochastic fluorescence off–on switches to support time‐resolved localization of single fluorophore. KRh s are functionalized into KRh‐MitoFix , KRh‐Mem and KRh‐Halo that demonstrate mitochondria, plasma membrane and fusion protein targeting ability, respectively. Consequently, these KRh probes demonstrate straightforward usage for super‐resolution imaging of these targets in live cells. Therefore, KRh s merit future development for fluorescence labeling and super‐resolution imaging in the NIR region.