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3D‐Non‐destructive Imaging through Heavy‐Metal Eosin Salt Contrast Agents
Author(s) -
Busse Madleen,
Marciniszyn Jaroslaw P.,
Ferstl Simone,
Kimm Melanie A.,
Pfeiffer Franz,
Gulder Tanja
Publication year - 2021
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202005203
Subject(s) - contrast (vision) , histology , contrast enhancement , materials science , fluorescein , biomedical engineering , chemistry , radiology , computer science , optics , medicine , pathology , fluorescence , physics , artificial intelligence , magnetic resonance imaging
Conventional histology is a destructive technique based on the evaluation of 2D slices of a 3D biopsy. By using 3D X‐ray histology these obstacles can be overcome, but their application is still restricted due to the inherently low attenuation properties of soft tissue. In order to solve this problem, the tissue can be stained before X‐ray computed tomography imaging (CT) to enhance the soft tissue X‐ray contrast. Evaluation of brominated fluorescein salts revealed a mutual influence of the number of bromine atoms and the cations applied on the achieved contrast enhancement. The dibromo fluorescein barium salt turned out to be the ideal X‐ray contrast agent, allowing for 3D imaging and subsequent complementing counterstaining applying standard histological techniques.