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Boronic Acid‐Mediated Activity Control of Split 10–23 DNAzymes
Author(s) -
Debiais Mégane,
Lelievre Amandine,
Vasseur JeanJacques,
Müller Sabine,
Smietana Michael
Publication year - 2021
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202004227
Subject(s) - deoxyribozyme , cleave , boronic acid , oligonucleotide , ribonucleotide , chemistry , ribozyme , combinatorial chemistry , biochemistry , rna , dna , nucleotide , gene
The 10–23 DNAzyme is an artificially developed Mg 2+ ‐dependent catalytic oligonucleotide that can cleave an RNA substrate in a sequence‐specific fashion. In this study, new split 10–23 DNAzymes made of two nonfunctional fragments, one of which carries a boronic acid group at its 5′ end, while the other has a ribonucleotide at its 3′ end, were designed. Herein it is demonstrated that the addition of Mg 2+ ions leads to assembly of the fragments, which in turn induces the formation of a new boronate internucleoside linkage that restores the DNAzyme activity. A systematic evaluation identified the best‐performing system. The results highlight key features for efficient control of DNAzyme activity through the formation of boronate linkages.