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Front Cover: Enhancing Robustness of Sortase A by Loop Engineering and Backbone Cyclization (Chem. Eur. J. 60/2020)
Author(s) -
Zou Zhi,
Mate Diana M.,
Nöth Maximilian,
Jakob Felix,
Schwaneberg Ulrich
Publication year - 2020
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202004038
Subject(s) - sortase a , sortase , front cover , robustness (evolution) , cover (algebra) , chemistry , protein engineering , loop (graph theory) , combinatorial chemistry , engineering , bacterial protein , biochemistry , mathematics , combinatorics , mechanical engineering , gene , enzyme
Sortase‐mediated ligation (sortagging) has emerged as one of the most popular methodologies for site‐specific modification of proteins. The cover image shows a loop engineering and subsequent head‐to‐tail backbone cyclization of Staphylococcus aureus sortase A (SaSrtA, the engineered loop and fused termini are highlighted in cyan). The reengineered SaSrtA has an enhanced robustness towards thermal and chemical denaturation. More information can be found in the Communication by U. Schwaneberg et al. on page 13568.