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Concentration‐Dependent Interactions of Amphiphilic PiB Derivative Metal Complexes with Amyloid Peptides Aβ and Amylin **
Author(s) -
Majdoub Saida,
Garda Zoltán,
Oliveira Alexandre C.,
Relich Inga,
Pallier Agnès,
Lacerda Sara,
Hureau Christelle,
Geraldes Carlos F. G. C.,
Morfin JeanFrançois,
Tóth Éva
Publication year - 2021
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202004000
Subject(s) - amylin , chemistry , thioflavin , peptide , amyloid (mycology) , biodistribution , amphiphile , surface plasmon resonance , linker , biophysics , stereochemistry , biochemistry , in vitro , organic chemistry , insulin , copolymer , islet , nanoparticle , nanotechnology , materials science , endocrinology , pathology , computer science , biology , operating system , polymer , inorganic chemistry , medicine , disease , alzheimer's disease
Abstract Metal chelates targeted to amyloid peptides are widely explored as diagnostic tools or therapeutic agents. The attachment of a metal complex to amyloid recognition units typically leads to a decrease in peptide affinity. We show here that by separating a macrocyclic GdL chelate and a PiB targeting unit with a long hydrophobic C10 linker, it is possible to attain nanomolar affinities for both Aβ 1‐40 ( K d =4.4 n m ) and amylin ( K d =4.5 n m ), implicated, respectively in Alzheimer's disease and diabetes. The Scatchard analysis of surface plasmon resonance data obtained for a series of amphiphilic, PiB derivative GdL complexes indicate that their Aβ 1‐40 or amylin binding affinity varies with their concentration, thus micellar aggregation state. The GdL chelates also affect peptide aggregation kinetics, as probed by thioflavin‐T fluorescence assays. A 2D NMR study allowed identifying that the hydrophilic region of Aβ 1‐40 is involved in the interaction between the monomer peptide and the Gd 3+ complex. Finally, ex vivo biodistribution experiments were conducted in healthy mice by using 111 In labeled analogues. Their pancreatic uptake, ∼3 %ID g −1 , is promising to envisage amylin imaging in diabetic animals.