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Cover Feature: Nucleotide‐Bearing Benzylidene‐Tetrahydroxanthylium Near‐IR Fluorophore for Sensing DNA Replication, Secondary Structures and Interactions (Chem. Eur. J. 52/2020)
Author(s) -
Kuba Miroslav,
Kraus Tomáš,
Pohl Radek,
Hocek Michal
Publication year - 2020
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202003781
Subject(s) - dna , nucleotide , fluorophore , dna polymerase , fluorescence , chemistry , biophysics , dna replication , microbiology and biotechnology , polymerase , rna , in vitro , computational biology , biology , biochemistry , gene , physics , quantum mechanics
A novel labeled nucleotide exerts very low near‐IR fluorescence in water but lights‐up (up to a 348‐fold increase) after polymerase incorporation to DNA. It can be used to directly visualize enzymatic DNA synthesis in vitro and the labeled DNA probes are also responsive to secondary structure changes or interactions with small molecules or proteins. Its application was demonstrated on real‐time PCR detection of RNA from SARS‐CoV‐2 virus. More information can be found in the Communication by M. Hocek et al. on page 11950.