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Strategies for 1 H‐Detected Dynamic Nuclear Polarization Magic‐Angle Spinning NMR Spectroscopy
Author(s) -
Concistré Maria,
Paul Subhradip,
Carravetta Marina,
Kuprov Ilya,
Williamson Philip T. F.
Publication year - 2020
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202003463
Subject(s) - magic angle spinning , spectroscopy , nuclear magnetic resonance spectroscopy , spinning , isotope , nuclear magnetic resonance , polarization (electrochemistry) , analytical chemistry (journal) , proton , chemistry , spectral line , materials science , magic angle , physics , nuclear physics , chromatography , astronomy , quantum mechanics , polymer chemistry
Combining dynamic nuclear polarization with proton detection significantly enhances the sensitivity of magic‐angle spinning NMR spectroscopy. Herein, the feasibility of proton‐detected experiments with slow (10 kHz) magic angle spinning was demonstrated. The improvement in sensitivity permits the acquisition of indirectly detected 14 N NMR spectra allowing biomolecular structures to be characterized without recourse to isotope labelling. This provides a new tool for the structural characterization of environmental and medical samples, in which isotope labelling is frequently intractable.