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Cover Feature: Ultrabright Terbium Nanoparticles for FRET Biosensing and in Situ Imaging of Epidermal Growth Factor Receptors (Chem. Eur. J. 64/2020)
Author(s) -
Charpentier Cyrille,
Cifliku Vjona,
Goetz Joan,
at Aline,
Cheig Clémence,
Cardoso Dos Santos Marcelina,
FrancésSoriano Laura,
Wong KaLeung,
Charbonnière Loïc J.,
Hildebrandt Niko
Publication year - 2020
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202003301
Subject(s) - bioconjugation , förster resonance energy transfer , nanoparticle , terbium , epidermal growth factor receptor , biosensor , chemistry , nanotechnology , biophysics , live cell imaging , luminescence , materials science , receptor , fluorescence , cell , biochemistry , biology , optoelectronics , physics , quantum mechanics
Novel trifunctional ligands enable simultaneous bioconjugation (e.g., to antibodies), attachment to nanoparticle surfaces, and photosensitization of Tb ions, affording ultrabright biofunctionalized Tb nanoparticles. These luminescent nanoparticle bioconjugates were successfully implemented in time‐gated FRET spectroscopy and luminescence microscopy for homogeneous in‐solution biosensing and live‐cell imaging of EGFR transmembrane receptors. More information can be found in the Full Paper by L. J. Charbonnière, N. Hildebrandt, et al. on page 14602.