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Cover Feature: A Systematic Study of Coumarin–Tetrazine Light‐Up Probes for Bioorthogonal Fluorescence Imaging (Chem. Eur. J. 44/2020)
Author(s) -
Galeta Juraj,
Dzijak Rastislav,
Obořil Jan,
Dračínský Martin,
Vrabel Milan
Publication year - 2020
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202003021
Subject(s) - bioorthogonal chemistry , tetrazine , fluorescence , coumarin , chemistry , combinatorial chemistry , reagent , cover (algebra) , biophysics , click chemistry , organic chemistry , biology , mechanical engineering , physics , engineering , quantum mechanics
Bioorthogonal fluorogenic reagents that produce after chemical activation fluorescent products represent superior probes for cellular imaging. Due to the improved signal‐to‐noise ratio, they can be applied in no‐wash fluorogenic labeling experiments. The present study reveals a set of coumarin‐tetrazine light‐up probes, which produce bright dyes in reaction with TCO or BCN dienophile‐tagged molecules and enable fast bioorthogonal labeling in live cells. More information can be found in the Full Paper by M. Vrabel et al. on page 9945.