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Structure–Activity Relationship Study of a Potent α‐Thrombin Binding Aptamer Incorporating Hexitol Nucleotides
Author(s) -
De Fenza Maria,
Eremeeva Elena,
Troisi Romualdo,
Yang Hui,
Esposito Anna,
Sica Filomena,
Herdewijn Piet,
D'Alonzo Daniele,
Guaragna Annalisa
Publication year - 2020
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202001504
Subject(s) - aptamer , nucleotide , oligonucleotide , thrombin , chemistry , biochemistry , stereochemistry , biology , microbiology and biotechnology , dna , platelet , gene , immunology
Abstract The replacement of one or more nucleotide residues in the potent α‐thrombin‐binding aptamer NU172 with hexitol‐based nucleotides has been devised to study the effect of these substitutions on the physicochemical and functional properties of the anticoagulant agent. The incorporation of single hexitol nucleotides at the T9 and G18 positions of NU172 substantially retained the physicochemical features of the parent oligonucleotide, as a result of the biomimetic properties of the hexitol backbone. Importantly, the NU172‐ T H 9 mutant exhibited a higher binding affinity toward human α‐thrombin than the native aptamer and an improved stability even after 24 h in 90 % human serum, with a significant increase in the estimated half‐life. The anticoagulant activity of the modified oligonucleotide was also found to be slightly preferable to NU172. Overall, these results confirm the potential of hexitol nucleotides as biomimetic agents, while laying the foundations for the development of NU172‐inspired α‐thrombin‐binding aptamers.