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One Terminal Guanosine Flip of Intramolecular Parallel G‐Quadruplex: Catalytic Enhancement of G‐Quadruplex/Hemin DNAzymes
Author(s) -
Cao Yanwei,
Li Wenjing,
Gao Tian,
Ding Pi,
Pei Renjun
Publication year - 2020
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202001462
Subject(s) - g quadruplex , deoxyribozyme , hemin , guanosine , intramolecular force , chemistry , terminal (telecommunication) , catalysis , stereochemistry , computer science , biochemistry , heme , dna , telecommunications , enzyme
Numerous studies have shown compelling evidence that incorporation of an inversion of polarity site (IPS) in G‐rich sequences can affect the topological and structural characteristics of G‐quadruplexes (G4s). Herein, the influence of IPS on the formation of a previously studied intramolecular parallel G4 of d(G 3 TG 3 TG 3 TG 3 ) (TTT) and its stacked higher‐order structures is explored. Insertion of 3′–3′ or 5′–5′ IPS did not change the parallel folding pattern of TTT. However, both the species and position of the IPS in TTT have a significant impact on the G4 stability and end‐stacking through the alteration of G4–G4 interfaces properties. The data demonstrate that one base flip in each terminal G‐tetrad can stabilize parallel G4s and facilitate intermolecular packing of monomeric G4s. Such modifications can also enhance the fluorescence and enzymatic performances by promoting interactions between parallel G4s with N ‐methyl mesoporphyrin IX (NMM) and hemin, respectively.