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Sensor Array Based Determination of Edman Degradated Amino Acids Using Poly( p ‐phenyleneethynylene)s
Author(s) -
Zhang Hao,
Wang Benhua,
Seehafer Kai,
Bunz Uwe H. F.
Publication year - 2020
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202001262
Subject(s) - edman degradation , amino acid , chemistry , chromatography , fluorescence , mass spectrometry , analytical chemistry (journal) , peptide sequence , biochemistry , physics , quantum mechanics , gene
A cross‐reactive optical sensor array based on poly( p ‐phenyleneethynylene)s (PPEs) determines Edman degraded amino acids. We report a sensor array composed of three anionic PPEs P1–P3 , and their electrostatic complexes with metal ions (Fe 2+ , Cu 2+ , Co 2+ ). We recorded distinct fluorescence intensity response patterns as “fingerprints” of this chemical tongue toward standard phenylthiohydantoin (PTH) amino acids—degradation products of the Edman process. These “fingerprints” were converted into canonical scores by linear discrimination analysis (LDA), which differentiates all of the PTH‐amino acids. This array discriminates PTH‐amino acid residues degraded from an oligopeptide through Edman sequencing. This approach is complementary to chromatography approaches which rely on mass spectrometry; our array offers the advantage of simplicity.