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Aqueous Synthesis of Triphenylphosphine‐Modified Gold Nanoparticles for Synergistic In Vitro and In Vivo Photothermal Chemotherapy
Author(s) -
Benyettou Farah,
Ramdas Nair Anjana,
Dho Yaereen,
Prakasam Thirumurugan,
Pasricha Renu,
Whelan Jamie,
Traboulsi Hassan,
Mazher Javed,
Sadler Kirsten C.,
Trabolsi Ali
Publication year - 2020
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202000216
Subject(s) - hela , aqueous solution , photothermal therapy , colloidal gold , photodegradation , in vivo , biophysics , nanoparticle , triphenylphosphine , chemistry , nuclear chemistry , intracellular , photochemistry , in vitro , materials science , nanotechnology , organic chemistry , biochemistry , catalysis , biology , photocatalysis , microbiology and biotechnology
Abstract Triphenylphosphine (TPP) surface‐functionalized and F‐108 Pluronic‐stabilized gold nanoparticles (F‐108@TPP‐AuNPs) have been synthesized through a one‐step approach, leading to well‐defined (9.6±1.6 nm) and water‐soluble nanoparticles by microwave heating an aqueous solution of TPP‐Au I Cl in the presence of a Pluronic polymer under basic conditions. TPP release was negligible under physiological conditions, but enhanced significantly at an acidic pH (5.4) mimicking that of a cancer cell. Laser irradiation (532 nm) raised the temperature of an aqueous solution of F‐108@TPP‐AuNPs to 51.7 °C within 5 min, confirming efficient light‐to‐heat conversion capabilities without significant photodegradation. TEM confirmed intracellular localization of F‐108@TPP‐AuNPs in the cytosol, endosomes and lysosomes of HeLa cells. F‐108@TPP‐AuNPs were well tolerated by HeLa cells and zebrafish embryos at ambient temperatures and became toxic upon heat activation, suggesting synergistic interactions between heat and cytotoxic action by TPP.