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Aggregation‐Induced Synergism by Hydrophobic‐Driven Self‐Assembly of Amphiphilic Oligonucleotides
Author(s) -
Yang Peng,
Peng Xin,
Yang Zezhou,
Chai Yaqin,
Yuan Ruo,
Liang Wenbin
Publication year - 2020
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.202000099
Subject(s) - amphiphile , oligonucleotide , chemistry , dna , combinatorial chemistry , hydrophobic effect , biophysics , micelle , fluorescence , reactivity (psychology) , monomer , nanotechnology , polymer , aqueous solution , biochemistry , materials science , copolymer , organic chemistry , biology , medicine , physics , alternative medicine , pathology , quantum mechanics
The evident contradiction between high local‐concentration‐based substrate reactivity and free‐diffusion‐based high reaction efficiency remains one of the important challenges in chemistry. Herein, we propose an efficient aggregation‐induced synergism through the hydrophobic‐driven self‐assembly of amphiphilic oligonucleotides to generate high local concentration whereas retaining high reaction efficiency through hydrophobic‐based aggregation, which is important for constructing efficient DNA nanomachines for ultrasensitive applications. MicroRNA‐155, used as a model, triggered strand displacement amplification of the DNA monomers on the periphery of the 3D DNA nanomachine and generated an amplified fluorescent response for its sensitive assay. The local concentration of substrates was increased by a factor of at least 9.0×10 5 through hydrophobic‐interaction‐based self‐assembly in comparison with the traditional homogeneous reaction system, achieving high local‐concentration‐based reactivity and free‐diffusion‐based enhanced reaction efficiency. As expected, the aggregation‐induced synergism by hydrophobic‐driven self‐assembly of amphiphilic oligonucleotides created excellent properties to generate a 3D DNA nanomachine with potential as an assay for microRNA‐155 in cells. Most importantly, this approach can be easily expanded for the bioassay of various biomarkers, such as nucleotides, proteins, and cells, offering a new avenue for simple and efficient applications in bioanalysis and clinical diagnosis.