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Self‐Assembly of DNA–Peptide Supermolecules: Coiled‐Coil Peptide Structures Templated by d ‐DNA and l ‐DNA Triplexes Exhibit Chirality‐Independent but Orientation‐Dependent Stabilizing Cooperativity
Author(s) -
Lou Chenguang,
Boesen Josephine Tuborg,
Christensen Niels Johan,
Sørensen Kasper K.,
Thulstrup Peter W.,
Pedersen Martin Nors,
Giralt Ernest,
Jensen Knud J.,
Wengel Jesper
Publication year - 2020
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201905636
Subject(s) - dna origami , cooperativity , dna , peptide , nucleic acid , chirality (physics) , peptide nucleic acid , coiled coil , helix (gastropod) , circular dichroism , molecular machine , template , chemistry , biophysics , nanotechnology , crystallography , materials science , biochemistry , biology , physics , ecology , chiral symmetry breaking , quantum mechanics , snail , nambu–jona lasinio model , quark
DNA nanostructures have been designed and used in many different applications. However, the use of nucleic acid scaffolds to promote the self‐assembly of artificial protein mimics is only starting to emerge. Herein five coiled‐coil peptide structures were templated by the hybridization of a d ‐DNA triplex or its mirror‐image counterpart, an l ‐DNA triplex. The self‐assembly of the desired trimeric structures in solution was confirmed by gel electrophoresis and small‐angle X‐ray scattering, and the stabilizing synergy between the two domains was found to be chirality‐independent but orientation‐dependent. This is the first example of using a nucleic acid scaffold of l ‐DNA to template the formation of artificial protein mimics. The results may advance the emerging POC‐based nanotechnology field by adding two extra dimensions, that is, chirality and polarity, to provide innovative molecular tools for rational design and bottom‐up construction of artificial protein mimics, programmable materials and responsive nanodevices.