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Intrinsic Turn‐On Response of Thioflavin T in Complexes
Author(s) -
Kung Jocky C. K.,
Vurgun Nesrin,
Chen JoAnn C.,
Nitz Mark,
Jockusch Rebecca A.
Publication year - 2020
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201905100
Subject(s) - thioflavin , chromophore , turn (biochemistry) , chemistry , intermolecular force , fluorescence , photochemistry , solvent , phase (matter) , organic chemistry , molecule , biochemistry , medicine , physics , disease , pathology , quantum mechanics , alzheimer's disease
The fluorescence enhancement (“turn‐on”) response of the amyloid‐sensing dye thioflavin T (ThT) is examined in vacuo, where solvent interactions are absent. Upon the complexation of ThT with a derivatized β‐cyclodextrin, heptakis‐[6‐deoxy‐6‐(3‐sulfanylpropanoic acid)]‐β‐cyclodextrin, turn‐on responses in both the gas phase and solution phase were observed. In contrast, turn‐on response was not detected when ThT was bound to gaseous cucurbit[7]uril or human telomeric DNA 22AG, whereas clear turn‐on response occurs in solution. The observed difference in turn‐on response in the gas phase emphasizes the key interplay between chromophore, host and solvent and demonstrates the utility of gas‐phase spectroscopy to tease out the balance among intermolecular forces driving the behavior of important chromophores in solution.