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Metal‐Free Photoinduced Atom Transfer Radical Polymerization for Highly Sensitive Detection of Lung Cancer DNA
Author(s) -
Liu Qianrui,
Jian Lihe,
Liu Ruiqian,
Yang Huaixia,
Kong Jinming,
Zhang Xueji
Publication year - 2020
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201904271
Subject(s) - photoinduced electron transfer , atom transfer radical polymerization , chemistry , photochemistry , detection limit , quenching (fluorescence) , eosin y , polymerization , biomolecule , fluorescence , electron transfer , radical , biosensor , combinatorial chemistry , catalysis , photocatalysis , polymer , organic chemistry , biochemistry , physics , chromatography , quantum mechanics
Convenient and sensitive detection of biomolecules is of great significance to disease diagnosis. In this work, a metal‐free photoinduced atom transfer radical polymerization (photoATRP) by a reductive quenching pathway as a novel strategy is applied to achieve lung cancer DNA detection. Thiolated PNA is exploited to specifically recognize target DNA, and the initiator of photoATRP is linked to the electrode surface via phosphate‐Zr 4+ ‐carboxylate. Under the excitation of blue light, the reductive quenching pathway is activated with eosin Y (EY) as photoredox catalyst and N , N , N ′, N ′′, N ′‐pentamethyldiethylenetriamine (PMDETA) as electron donor, and numerous polymeric chains are formed. Under optimal conditions, the linear range of this strategy is from 0.1 p m to 10 n m ( R 2 =0.989) with a limit of detection (LOD) of 1.4 f m (14 zmol in 10 μL). The variety of possible light sources for photoATRP and simple operation endow this biosensor with great potential for practical applications.