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A DNA Switch for Detecting Single Nucleotide Polymorphism within a Long DNA Sequence Under Denaturing Conditions
Author(s) -
Zhang Wenqing,
Li Jiuxing,
Salena Bruno,
Li Yingfu
Publication year - 2020
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201903536
Subject(s) - dna , nucleotide , dna nanoball sequencing , genetics , dna sequencing , single nucleotide polymorphism , biology , base pair , snp , sequence (biology) , nucleic acid sequence , computational biology , microbiology and biotechnology , base sequence , gene , genotype , genomic library
DNA detection is usually conducted under nondenaturing conditions to favor the formation of Watson–Crick base‐paring interactions. However, although such a setting is excellent for distinguishing a single‐nucleotide polymorphism (SNP) within short DNA sequences (15–25 nucleotides), it does not offer a good solution to SNP detection within much longer sequences. Here we report on a new detection method capable of detecting SNP in a DNA sequence containing 35–90 nucleotides. This is achieved through incorporating into the recognition DNA sequence a previously discovered DNA molecule that forms a stable G‐quadruplex in the presence of 7 molar urea, a known condition for denaturing DNA structures. The systems are configured to produce both colorimetric and fluorescent signals upon target binding.