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Disulfide‐Containing Detergents (DCDs) for the Structural Biology of Membrane Proteins
Author(s) -
Xue Dongxiang,
Xu Tiandan,
Wang Huixia,
Wu Meng,
Yuan Ya,
Wang Wei,
Tan Qiwen,
Zhao Fei,
Zhou Fang,
Hu Tao,
Jiang Zhongxing,
Liu ZhiJie,
Zhao Suwen,
Liu Dongsheng,
Wüthrich Kurt,
Tao Houchao
Publication year - 2019
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201903190
Subject(s) - disulfide bond , micelle , solubilization , membrane protein , chemistry , structural biology , membrane , biochemistry , organic chemistry , aqueous solution
Disulfide‐containing detergents (DCDs) are introduced, which contain a disulfide bond in the hydrophobic tail. DCDs form smaller micelles than corresponding detergents with linear hydrocarbon chains, while providing good solubilization and reconstitution of membrane proteins. The use of this new class of detergents in structural biology is illustrated with solution NMR spectra of the human G protein‐coupled receptor A 2A AR, which is an α‐helical protein, and the β‐barrel protein OmpX from E. coli .