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Cover Feature: Fluorescent Turn‐On Probes for the Development of Binding and Hydrolytic Activity Assays for mRNA Cap‐Recognizing Proteins (Chem. Eur. J. 27/2019)
Author(s) -
Kasprzyk Renata,
Starek Beata J.,
Ciechanowicz Sylwia,
Kubacka Dorota,
Kowalska Joanna,
Jemielity Jacek
Publication year - 2019
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201901431
Subject(s) - pyrene , nucleotide , cleavage (geology) , chemistry , eif4e , enzyme , biochemistry , fluorescence , messenger rna , translation (biology) , eukaryotic translation , microbiology and biotechnology , stereochemistry , biology , gene , organic chemistry , paleontology , physics , quantum mechanics , fracture (geology)
7‐Methylguanine nucleotides labelled with pyrene were found to behave as sensitive turn‐on probes upon enzymatic cleavage or binding to specific proteins. This observation served as the basis for developing binding‐ and hydrolytic‐activity assays in a 96‐well format for two therapeutically‐relevant 7‐methylguanosine cap recognising proteins: translation initiation factor (eIF4E) and decapping enzyme (DcpS). A complex of eIF4E with pyrene labelled nucleotide is depicted on the cover. More information can be found in the Full Paper by J. Kowalska, J. Jemielity, et al. on page 6728.