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Cover Feature: Structural and Kinetic Profiling of Allosteric Modulation of Duplex DNA Induced by DNA‐Binding Polyamide Analogues (Chem. Eur. J. 11/2019)
Author(s) -
Aman Khalid,
Padroni Giacomo,
Parkinson John A.,
Welte Thomas,
Burley Glenn A.
Publication year - 2019
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201900278
Subject(s) - allosteric regulation , dna , steric effects , chemistry , duplex (building) , polyamide , biophysics , stereochemistry , biochemistry , computational biology , biology , receptor , organic chemistry
DNA‐binding polyamides are molecules that hold great promise as regulators of gene expression. One of the challenges in their therapeutic development is understanding the molecular determinants governing their DNA‐binding profile. This article describes a new biophysical technique (switchSENSE®) to determine the DNA‐binding parameters of polyamide analogues. NMR structural analysis shows that steric bulk distal to the DNA‐binding face plays an influential role in allosteric modulation of the DNA duplex. More information can be found in the Full Paper by T. Welte, G. A. Burley et al. on page 2757.