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Programming Rotary Motions with a Hexagonal DNA Nanomachine
Author(s) -
Yang Yangyang,
Zhang Shiwei,
Yao Shengtao,
Pan Rizhao,
Hidaka Kumi,
Emura Tomoko,
Fan Chunhai,
Sugiyama Hiroshi,
Xu Yufang,
Endo Masayuki,
Qian Xuhong
Publication year - 2019
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201900221
Subject(s) - nanodevice , molecular machine , horseradish peroxidase , glucose oxidase , dna origami , nanotechnology , rotation (mathematics) , dna , chemistry , work (physics) , molecular motor , nanostructure , materials science , biophysics , biosensor , enzyme , biochemistry , mechanical engineering , computer science , engineering , artificial intelligence , biology
Biological macromolecular machines perform impressive mechanical movements. F‐adenosine triphosphate (ATP) synthase uses a proton gradient to generate ATP through mechanical rotations. Here, a programmed hexagonal DNA nanomachine, in which a three‐armed DNA nanostructure (TAN) can perform stepwise rotations in the confined nanospace powered by DNA fuels, is demonstrated. The movement of TAN can precisely go through a 60° rotation, which is confirmed by atomic force microscopy, and each stepwise directional rotating is monitored by fluorescent measurements. Moreover, the rotary nanomachine is used to spatially organize cascade enzymes: glucose oxidase (GOx) and horseradish peroxidase (HRP) in four different arrangements. The multistep regulations of the biocatalytic activities are achieved by employing TAN rotations. This work presents a new prototype of rotary nanodevice with both angular and directional control, and provides a nanoscale mechanical engineering platform for the reactive molecular components, demonstrating that DNA‐based framework may have significant roles in futuristic nanofactory construction.

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