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Comparative Study of Photoswitchable Zinc‐Finger Domain and AT‐Hook Motif for Light‐Controlled Peptide–DNA Binding
Author(s) -
Murawska Gosia M.,
Poloni Claudia,
Simeth Nadja A.,
Szymanski Wiktor,
Feringa Ben L.
Publication year - 2019
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201900090
Subject(s) - dna , peptide , zinc finger , biophysics , microbiology and biotechnology , dna replication , dna binding domain , chemistry , protein–dna interaction , biochemistry , biology , dna binding protein , transcription factor , gene
DNA–peptide interactions are involved in key life processes, including DNA recognition, replication, transcription, repair, organization, and modification. Development of tools that can influence DNA–peptide binding non‐invasively with high spatiotemporal precision could aid in determining its role in cells and tissues. Here, the design, synthesis, and study of photocontrolled tools for sequence‐specific small peptide‐DNA major and minor groove interactions are reported, shedding light on DNA binding by transcriptionally active peptides. In particular, photoswitchable moieties were implemented in the peptide backbone or turn region. In each case, DNA binding was affected by photochemical isomerization, as determined in fluorescent displacement assays on model DNA strands, which provides promising tools for DNA modulation.