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Frontispiece: DNAzyme Feedback Amplification: Relaying Molecular Recognition to Exponential DNA Amplification
Author(s) -
Liu Meng,
Yin Qingxin,
McConnell Erin M.,
Chang Yangyang,
Brennan John D.,
Li Yingfu
Publication year - 2018
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201881861
Subject(s) - deoxyribozyme , nucleic acid , rolling circle replication , dna , multiple displacement amplification , loop mediated isothermal amplification , biology , computational biology , applications of pcr , analyte , polymerase chain reaction , microbiology and biotechnology , computer science , polymerase , chemistry , genetics , dna extraction , digital polymerase chain reaction , gene , chromatography
A simple but powerful isothermal DNA amplification method, termed DNAzyme feedback amplification (DFA) capable of relaying molecular recognition to exponential DNA amplification is presented. The method incorporates both an RNA‐cleaving DNAzyme (RCD) and rolling circle amplification (RCA) carried out by a special DNA polymerase using a circular DNA template. This method can be used for the detection of both nucleic acid based targets and non‐nucleic acid analytes. The conceptual framework of the feedback amplification approach, the essential features of this method, and the remaining challenges and possible solutions are discussed on page 4473 ff.