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[Ru(dcbpy) 2 dppz] 2+ /Fullerene Cosensitized PTB7‐Th for Ultrasensitive Photoelectrochemical MicroRNA Assay
Author(s) -
Xia LingYing,
Zheng YingNing,
Liang WenBin,
Li MengJie,
Hu Tao,
Yuan Ruo,
Chai YaQin
Publication year - 2019
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201806005
Subject(s) - photocurrent , detection limit , ruthenium , materials science , fullerene , biosensor , chemistry , photochemistry , nuclear chemistry , analytical chemistry (journal) , nanotechnology , optoelectronics , organic chemistry , chromatography , catalysis
A new cosensitization photoelectrochemical (PEC) strategy was established by using a donor–acceptor‐type photoactive material, poly{4,8‐bis[5‐(2‐ethylhexyl)thiophen‐2‐yl]benzo[1,2‐ b :4,5‐ b ′]dithiophene‐2,6‐diyl‐ alt ‐3‐fluoro‐2‐[(2‐ethylhexyl)carbonyl]thieno[3,4‐ b ]thiophene‐4,6‐diyl} (PTB7‐Th), as a signal indicator, which was cosensitized with bis(4,4′dicarboxyl‐2,2′‐bipyridyl)(4,5,9,14‐tetraazabenzo[ b ]triphenylene)ruthenium(II) ([Ru(dcbpy) 2 dppz] 2+ ) embedded in the grooves of the DNA duplex and fullerene (nano‐C 60 ) immobilized on the surface of DNA nanoflowers for microRNA assay. [Ru(dcbpy) 2 dppz] 2+ and nano‐C 60 could effectively enhance the photoelectric conversion efficiency (PCE) of PTB7‐Th as a result of well‐matched energy levels among nano‐C 60 , [Ru(dcbpy) 2 dppz] 2+ and PTB7‐Th, leading to a clearly enhanced photocurrent signal. Meanwhile, a target recycling magnification technique based on duplex‐specific nuclease was applied in this work to obtain higher detection sensitivity. The proposed biosensor demonstrated excellent analytical properties within a linear detection range of 2.5 f m to 2.5 n m and a limit of detection down to 0.83 f m . Impressively, this cosensitization PEC strategy offers an effective and convenient avenue to significantly improve the PCE of a photoactive material, resulting in a remarkably improved photocurrent signal for ultrasensitive and highly accurate detection of various targets.