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Light‐Controlled Chemoenzymatic Immobilization of Proteins towards Engineering of Bioactive Papers
Author(s) -
Hilberg Valentina,
Avrutina Olga,
Ebenig Aileen,
Yanakieva Desislava,
Meckel Tobias,
Biesalski Markus,
Kolmar Harald
Publication year - 2019
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201804889
Subject(s) - sortase , modular design , covalent bond , combinatorial chemistry , computer science , protein engineering , sortase a , chemistry , nanotechnology , computational biology , biochemistry , materials science , bacterial protein , enzyme , biology , organic chemistry , programming language , gene
Efficient and reliable methods for the generation of bioactive papers are of growing interest in relation to point‐of‐care testing devices that do not require extensive analytical equipment. Herein, we report the immobilization of functional proteins on paper fibers using a modular chemoenzymatic approach. The synthetic strategy relies on a combination of highly efficient spatially controllable photo‐triggered cycloaddition followed by site‐specific sortase A‐catalyzed transamidation. This site‐directed and regiospecific method has allowed unidirectional and covalent immobilization of several proteins displaying different functional properties, with ramifications for application in paper‐based diagnostics.