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Light‐Induced Activation of c‐Met Signalling by Photocontrolled DNA Assembly
Author(s) -
Chen Shan,
Li Jingying,
Liang Hong,
Lin XiaHui,
Li Juan,
Yang HuangHao
Publication year - 2018
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201803868
Subject(s) - linker , microbiology and biotechnology , dna , signalling , signal transducing adaptor protein , signal transduction , chemistry , receptor , transduction (biophysics) , biophysics , biology , biochemistry , computer science , operating system
Optical manipulation appears to be a powerful tool for spatiotemporally controlling a variety of cellular functions. Herein, a photocontrolled DNA assembly approach is described which enables light‐induced activation of cellular signal transduction by triggering protein dimerization (c‐Met signalling in this case). Three kinds of DNA probes are designed, including a pair of receptor recognition probes with adaptors and a blocker probe with a photocleavable linker (PC‐linker). By implementing PC‐linkers in blocker probes, the designed DNA probes response to light irradiation, which then induces the assembly of receptor recognition probes through adaptor complementing. Consequently, light‐mediated DNA assembly promotes the dimerization of c‐Met receptors, resulting in activation of c‐Met signalling. It is demonstrated that the proposed photocontrolled DNA assembly approach is effective for regulating c‐Met signalling and modulating cellular behaviours, such as cell proliferation and migration. Therefore, this simple approach may offer a promising strategy to manipulate cell signalling pathways precisely in living cells.