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Luminescence from Lanthanide(III) Ions Bound to the Glycocalyx of Chinese Hamster Ovary Cells
Author(s) -
ArppeTabbara Riikka,
CarroTemboury Miguel R.,
Hempel Casper,
Vosch Tom,
Sørensen Thomas Just
Publication year - 2018
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201802799
Subject(s) - europium , chinese hamster ovary cell , terbium , lanthanide , luminescence , ion , chemistry , microprobe , emission spectrum , materials science , spectral line , physics , optoelectronics , biochemistry , mineralogy , receptor , organic chemistry , astronomy
Lanthanide(III) ions bind to the glycocalyx of Chinese Hamster Ovary (CHO) cells and give rise to a unique luminescent fingerprint. Following direct excitation of terbium(III) and europium(III) ions in the visible part of the spectrum, we are able to collect emission spectra pixel‐by‐pixel in images of CHO cells. Following data analysis that removes the background signal, the fine structure of the europium(III) luminescence indicate that the lanthanide(III) ions are bound to a single structure of the CHO cell glycocalyx. This was deduced from the fact that the structure‐sensitive emission spectrum of europium is unchanged throughout the investigated samples.