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Star‐Like Glycosaminoglycans with Superior Bioactivity Assemble with Proteins into Microfibers
Author(s) -
NovoaCarballal Ramon,
Carretero Agatha,
Pacheco Raul,
Reis Rui L.,
Pashkuleva Iva
Publication year - 2018
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201802243
Subject(s) - glycosaminoglycan , microfiber , chemistry , chondroitin sulfate , fibroblast growth factor , biophysics , chondroitin , growth factor , tissue engineering , biochemistry , biology , organic chemistry , receptor , genetics
Here it is shown that glycosaminoglycans (GAGs) with high molecular weight can be grafted via their reducing end on hyperbranched synthetic cores by oxime condensation without the need of any previous functionalisation of the polysaccharide. The versatility of this reaction is demonstrated by the use of hyaluronan, chondroitin sulfate and heparin with up to 60 sugar units. The isothermal calorimetry analysis demonstrated that the generated star‐like glycopolymers have superior bioactivity. Moreover, when mixed with positively charged proteins (e.g., fibroblast growth factor‐2, FGF‐2) they form microfiber structures instead of the spherical nanocomplexes described for linear GAGs. The results suggest that the described star‐like GAG are closer mimics of the proteoglycans at the structural and functional level and therefore have huge potential in the development of tissue engineering platforms and therapeutics by modulating the activity and presentation of various proteins such as growth factors.