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Cover Feature: Fluorescence Redox Blinking Adaptable to Structural Analysis of Nucleic Acids (Chem. Eur. J. 26/2018)
Author(s) -
Miyata Takafumi,
Shimada Naohiko,
Maruyama Atsushi,
Kawai Kiyohiko
Publication year - 2018
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201800629
Subject(s) - fluorophore , fluorescence , dna , redox , biophysics , nucleic acid , chemistry , duplex (building) , rna , biochemistry , biology , physics , optics , organic chemistry , gene
By designing patterns of fluorescence blinking , that is, fluctuation in fluorescence intensity, to be affected by the microenvironment around the fluorophore, various fluorophores can be turned into environment‐sensitive probes. Nucleice acids structures, such as single strand, B‐form DNA/DNA duplex, and A‐like DNA/RNA duplex can be discriminated by monitoring the blinking patterns. This method, termed Kinetic Analysis based on the Control of fluorescence Blinking utilizing the redox reactions (rKACB), is also adaptable to investigate antigen–antibody interactions at the single‐molecule level. More information can be found in the Full Paper by A. Maruyama, K. Kawai et al. on page 6755.