Premium
Frontispiece: Ratiometric Fluorescent Probes for the Detection of Reactive Oxygen Species
Author(s) -
Andina Diana,
Leroux JeanChristophe,
Luciani Paola
Publication year - 2017
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201785562
Subject(s) - fluorescence , reactive oxygen species , analyte , oxygen , chemistry , nanotechnology , signal (programming language) , biophysics , analytical chemistry (journal) , biological system , materials science , computer science , biology , optics , biochemistry , environmental chemistry , physics , chromatography , organic chemistry , programming language
Ratiometric fluorescent probes offer an elegant option to detect analytes of interest and to normalize the signal of short‐lived species such as reactive oxygen species (ROS) to a second fluorescence emission. The ratio of the two signals becomes thus independent of the sensor concentration and minimizes artefacts and intensity fluctuations. A summary of the advances made in the field is presented, giving an overview of current ratiometric fluorescent probes for ROS and critically discussing selected approaches, with a special focus on their application in cell cultures and in vivo systems. For more details, see the Review by J.‐C. Leroux, P. Luciani and D. Andina on page 13549 ff.