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Frontispiece: Bifunctional Diaminoterephthalate Fluorescent Dye as Probe for Cross‐Linking Proteins
Author(s) -
Wallisch Melanie,
Sulmann Stefan,
Koch KarlWilhelm,
Christoffers Jens
Publication year - 2017
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201782763
Subject(s) - fluorescence , bifunctional , recoverin , rhodopsin , chemistry , maleimide , bacteriorhodopsin , biophysics , retinal , biochemistry , biology , membrane , physics , optics , organic chemistry , catalysis
How “Link” links two proteins with a fluorescent probe is shown in the Full Paper by J. Christoffers et al. on page 6535 ff. Diaminoterephthalates are functional fluorescent dyes which increase their quantum yield upon reaction with a molecular target. Such a “turn‐on probe” with maleimide and cyclooctyne reactive sites is used to link the retinal Ca 2+ sensor recoverin with its target enzyme rhodopsin kinase. The Frontispiece graphic is inspired by the character “Link” in the fantasy game “The Legend of Zelda”.