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Identification of the Minimal Glycotope of Streptococcus pneumoniae 7F Capsular Polysaccharide using Synthetic Oligosaccharides
Author(s) -
Ménová Petra,
Sella Mauro,
Sellrie Katrin,
Pereira Claney L.,
Seeberger Peter H.
Publication year - 2018
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201705379
Subject(s) - streptococcus pneumoniae , polysaccharide , microbiology and biotechnology , identification (biology) , chemistry , biology , biochemistry , antibiotics , botany
Streptococcus pneumoniae causes life‐threatening diseases including meningitis, pneumonia and sepsis. Existing glycoconjugate vaccines based on purified capsular polysaccharides are widely used and help to prevent millions of deaths every year. Herein, the total syntheses of oligosaccharides resembling portions of the S. pneumoniae serotype 7F (ST7F) capsular polysaccharide repeating unit are reported. To define minimal glycan epitopes, glycan microarrays containing the synthetic oligosaccharides were used to screen human reference serum and revealed that both side chains of the ST7F play a key role in antigen recognition. The identification of protective minimal epitopes is vital to design efficient semi‐ and fully‐synthetic glycoconjugate vaccines.