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Interrogating Protein Phosphatases with Chemical Activity Probes
Author(s) -
Casey Garrett R.,
Stains Cliff I.
Publication year - 2018
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201705194
Subject(s) - phosphatase , covalent bond , enzyme , biochemistry , chemistry , chemical biology , computational biology , biology , organic chemistry
Protein phosphatases, while long overlooked, have recently become appreciated as drivers of both normal‐ and disease‐associated signaling events. As a result, the spotlight is now turning torwards this enzyme family and efforts geared towards the development of modern chemical tools for studying these enzymes are well underway. This Minireview focuses on the evolution of chemical activity probes, both optical and covalent, for the study of protein phosphatases. Small‐molecule probes, global monitoring of phosphatase activity through the use of covalent modifiers, and targeted fluorescence‐based activity probes are discussed. We conclude with an overview of open questions in the field and highlight the potential impact of chemical tools for studying protein phosphatases.