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First Structure of a Designed Minor Groove Binding Heterocyclic Cation that Specifically Recognizes Mixed DNA Base Pair Sequences
Author(s) -
Harika Narinder K.,
Germann Markus W.,
Wilson W. David
Publication year - 2017
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201704563
Subject(s) - dna , base pair , minor groove , sequence (biology) , molecule , amide , molecular recognition , chemistry , stereochemistry , molecular dynamics , molecular model , computational biology , resolution (logic) , crystallography , biology , computational chemistry , biochemistry , computer science , artificial intelligence , organic chemistry
The high‐resolution NMR structure of the first heterocyclic, non‐amide, organic cation that strongly and selectively recognizes mixed AT/GC bp (bp=base pair) sequences of DNA in a 1:1 complex is described. Compound designs of this type provide essential methods for control of functional, non‐genomic DNA sequences and have broad cell uptake capability, based on studies from animals to humans. The high‐resolution structural studies described in this report are essential for understanding the molecular basis for the sequence‐specific binding as well as for new ideas for additional compound designs for sequence‐specific recognition. The molecular features, in this report, explain the mechanism of recognition of both A⋅T and G⋅C bps and are an interesting molecular recognition story. Examination of the experimental structure and the NMR restrained molecular dynamics model suggests that recognition of the G⋅C base pair involves two specific H‐bonds. The structure illustrates a wealth of information on different DNA interactions and illustrates an interfacial water molecule that is a key component of the complex.