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DNA Hydrogel with Tunable pH‐Responsive Properties Produced by Rolling Circle Amplification
Author(s) -
Xu Wanlin,
Huang Yishun,
Zhao Haoran,
Li Pan,
Liu Guoyuan,
Li Jing,
Zhu Chengshen,
Tian Leilei
Publication year - 2017
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201704390
Subject(s) - self healing hydrogels , rolling circle replication , oligonucleotide , dna , template , polymer , materials science , nanotechnology , chemistry , polymer chemistry , biochemistry , dna replication , composite material
Recently, smart DNA hydrogels, which are generally formed by the self‐assembly of oligonucleotides or through the cross‐linking of oligonucleotide–polymer hybrids, have attracted tremendous attention. However, the difficulties of fabricating DNA hydrogels limit their practical applications. We report herein a novel method for producing pH‐responsive hydrogels by rolling circle amplification (RCA). In this method, pH‐sensitive cross‐linking sites were introduced into the polymeric DNA chains during DNA synthesis. As the DNA sequence can be precisely defined by its template, the properties of such hydrogels can be finely tuned in a very facile way through template design. We have investigated the process of hydrogel formation and pH‐responsiveness to provide rationales for functional hydrogel design based on the RCA reaction.