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A Microfluidic Device for Rapid Screening of E. coli O157:H7 Based on IFAST and ATP Bioluminescence Assay for Water Analysis
Author(s) -
Ngamsom Bongkot,
Truyts Alma,
Fourie Louis,
Kumar Shavon,
Tarn Mark D.,
Iles Alexander,
Moodley Klariska,
Land Kevin J.,
Pamme Nicole
Publication year - 2017
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201703487
Subject(s) - bioluminescence , escherichia coli , chromatography , luciferin , luciferase , filtration (mathematics) , chemistry , adenosine triphosphate , microfluidics , effluent , microbiology and biotechnology , biology , biochemistry , nanotechnology , materials science , environmental engineering , statistics , mathematics , transfection , engineering , gene
We present a simple microfluidic system for rapid screening of Escherichia coli ( E. coli ) O157:H7 employing the specificity of immunomagnetic separation (IMS) via immiscible filtration assisted by surface tension (IFAST), and the sensitivity of the subsequent adenosine triphosphate (ATP) assay by the bioluminescence luciferin/luciferase reaction. The developed device was capable of detecting E. coli O157:H7 from just 6 colony forming units (CFU) in 1 mL spiked buffer within 20 min. When tested with wastewater discharged effluent samples, without pre‐concentration, the device demonstrated the ability to detect 10 4 CFU per mL seeded; suggesting great potential for point‐of‐need microbiological water quality monitoring.