A Microfluidic Device for Rapid Screening of  E. coli  O157:H7 Based on IFAST and ATP Bioluminescence Assay for Water Analysis | Zendy

Ngamsom Bongkot | Zendy; Truyts Alma | Zendy; Fourie Louis | Zendy; Kumar Shavon | Zendy; Tarn Mark D. | Zendy; Iles Alexander | Zendy; Moodley Klariska | Zendy; Land Kevin J. | Zendy; Pamme Nicole | Zendy

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A Microfluidic Device for Rapid Screening of E. coli O157:H7 Based on IFAST and ATP Bioluminescence Assay for Water Analysis

Author(s) -

Ngamsom Bongkot,

Truyts Alma,

Fourie Louis,

Kumar Shavon,

Tarn Mark D.,

Iles Alexander,

Moodley Klariska,

Land Kevin J.,

Pamme Nicole

Publication year - 2017

Publication title -

chemistry – a european journal

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.687

H-Index - 242

eISSN - 1521-3765

pISSN - 0947-6539

DOI - 10.1002/chem.201703487

Subject(s) - bioluminescence , microfluidics , microbiology and biotechnology , chemistry , biology , biochemistry , nanotechnology , materials science

We present a simple microfluidic system for rapid screening of Escherichia coli ( E. coli ) O157:H7 employing the specificity of immunomagnetic separation (IMS) via immiscible filtration assisted by surface tension (IFAST), and the sensitivity of the subsequent adenosine triphosphate (ATP) assay by the bioluminescence luciferin/luciferase reaction. The developed device was capable of detecting E. coli O157:H7 from just 6 colony forming units (CFU) in 1 mL spiked buffer within 20 min. When tested with wastewater discharged effluent samples, without pre‐concentration, the device demonstrated the ability to detect 10 4 CFU per mL seeded; suggesting great potential for point‐of‐need microbiological water quality monitoring.

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