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Anomeric 2′‐Deoxycytidines and Silver Ions: Hybrid Base Pairs with Greatly Enhanced Stability and Efficient DNA Mismatch Detection with α‐dC
Author(s) -
Guo Xiurong,
Seela Frank
Publication year - 2017
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201703017
Subject(s) - base pair , dna , ion , thermal stability , duplex (building) , chemistry , nucleotide , crystallography , materials science , gene , biochemistry , organic chemistry
α‐ d ‐Nucleosides are rare in nature but can develop fascinating properties when incorporated into DNA. This work reports on the first silver‐mediated base pair constructed from two anomeric nucleosides: α‐dC and β‐dC. The hybrid base pair was integrated into the DNA and DNA/RNA double helix. A 12‐mer duplex with α‐dC and β‐dC pair exhibits a higher thermal stability ( T m =43 °C) than that incorporating the β‐dC–Ag + –β‐dC homo pair ( T m =34 °C). Furthermore, α‐dC shows excellent mismatch discrimination for DNA single nucleotide polymorphism (SNP). All four SNPs were identified on the basis of large T m value differences measured in the presence of silver ions. High resolution melting was not required.