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Preparation of Robust Metal‐Free Magnetic Nanoemulsions Encapsulating Low‐Molecular‐Weight Nitroxide Radicals and Hydrophobic Drugs Directed Toward MRI‐Visible Targeted Delivery
Author(s) -
Nagura Kota,
Takemoto Yusa,
Moronaga Satori,
Uchida Yoshiaki,
Shimono Satoshi,
Shiino Akihiko,
Tanigaki Kenji,
Amano Tsukuru,
Yoshino Fumi,
Noda Yohei,
Koizumi Satoshi,
Komatsu Naoki,
Kato Tatsuhisa,
Yamauchi Jun,
Tamura Rui
Publication year - 2017
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201702785
Subject(s) - dynamic light scattering , drug delivery , chemistry , nitroxide mediated radical polymerization , nanoparticle , nanomedicine , radical , materials science , magnetic nanoparticles , nuclear chemistry , chemical engineering , nanotechnology , organic chemistry , polymerization , radical polymerization , polymer , engineering
With a view to developing a theranostic nanomedicine for targeted drug delivery systems visible by magnetic resonance (MR) imaging, robust metal‐free magnetic nanoemulsions (mean particle size less than 20 nm) consisting of a biocompatible surfactant and hydrophobic, low molecular weight 2,2,5‐trimethyl‐5‐(4‐alkoxy)phenylpyrrolidine‐ N ‐oxyl radicals were prepared in pH 7.4 phosphate‐buffered saline (PBS). The structure of the nanoemulsions was characterized by electron paramagnetic resonance spectroscopy, and dynamic light scattering and small‐angle neutron‐scattering measurements. The nanoemulsions showed high colloidal stability, low cytotoxicity, enough reduction resistance to excess ascorbic acid, and sufficient contrast enhancement in the proton longitudinal relaxation time ( T 1 ) weighted MR images in PBS in vitro (and preliminarily in vivo). Furthermore, the hydrophobic anticancer drug paclitaxel could be encapsulated inside the nanoparticles, and the resulting paclitaxel‐loaded nanoemulsions were efficiently incorporated into HeLa cells to suppress cell growth.