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Preparation, Single‐Molecule Manipulation, and Energy Transfer Investigation of a Polyfluorene‐ graft ‐DNA polymer
Author(s) -
Madsen Mikael,
Christensen Rasmus S.,
Krissanaprasit Abhichart,
Bakke Mette R.,
Riber Camilla F.,
Nielsen Karina S.,
Zelikin Alexander N.,
Gothelf Kurt V.
Publication year - 2017
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201702780
Subject(s) - polyfluorene , conjugated system , polymer , materials science , nanotechnology , dna , förster resonance energy transfer , molecule , fluorescence , chemistry , organic chemistry , optics , physics , biochemistry , composite material
Conjugated polymers have been intensively studied due to their unique optical and electronic properties combined with their physical flexibility and scalable bottom up synthesis. Although the bulk qualities of conjugated polymers have been extensively utilized in research and industry, the ability to handle and manipulate conjugated polymers at the nanoscale lacks significantly behind. Here, the toolbox for controlled manipulation of conjugated polymers was expanded through the synthesis of a polyfluorene‐DNA graft‐type polymer ( poly(F‐DNA) ). The polymer possesses the characteristics associated with the conjugated polyfluorene backbone, but the protruding single‐stranded DNA provides the material with an exceptional addressability. This study demonstrates controlled single‐molecule patterning of poly(F‐DNA) , as well as energy transfer between two different polymer–DNA conjugates. Finally, highly efficient DNA‐directed quenching of polyfluorene fluorescence was shown.