Premium
Mechanisms of the Diterpene Cyclases β‐Pinacene Synthase from Dictyostelium discoideum and Hydropyrene Synthase from Streptomyces clavuligerus
Author(s) -
Rinkel Jan,
Rabe Patrick,
Chen Xinlu,
Köllner Tobias G.,
Chen Feng,
Dickschat Jeroen S.
Publication year - 2017
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201702704
Subject(s) - streptomyces clavuligerus , dictyostelium discoideum , diterpene , atp synthase , biochemistry , streptomyces , dictyostelium , stereochemistry , slime mold , biology , biosynthesis , chemistry , actinomycetales , enzyme , bacteria , genetics , gene
Two diterpene cyclases, one from the social amoeba Dictyostelium discoideum and the other from the bacterium Streptomyces clavuligerus , with products containing a Z ‐configured double bond between the original C2 and C3 of geranylgeranyl diphosphate, were extensively investigated for their mechanisms through isotopic labelling experiments. The participation of geranyllinalyl diphosphate, in analogy to the role of linalyl and nerolidyl diphosphate for mono‐ and sesquiterpene biosynthesis, as an intermediate towards diterpenes with a Z ‐configured C2=C3 double bond is discussed.