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Photo‐Induced Click Chemistry for DNA Surface Structuring by Direct Laser Writing
Author(s) -
Kerbs Antonina,
Mueller Patrick,
Kaupp Michael,
Ahmed Ishtiaq,
Quick Alexander S.,
Abt Doris,
Wegener Martin,
Niemeyer Christof M.,
BarnerKowollik Christopher,
Fruk Ljiljana
Publication year - 2017
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201700673
Subject(s) - dna , click chemistry , oligonucleotide , chemistry , cycloaddition , laser , aqueous solution , resolution (logic) , yield (engineering) , fabrication , nanotechnology , combinatorial chemistry , materials science , organic chemistry , optics , biochemistry , computer science , physics , artificial intelligence , metallurgy , catalysis , medicine , alternative medicine , pathology
Oligonucleotides containing photo‐caged dienes were prepared and shown to react quantitatively in a light‐induced Diels–Alder cycloaddition with functional maleimides in aqueous solution within minutes. Due to its high yield and fast rate, the reaction was exploited for DNA surface patterning with sub‐micrometer resolution employing direct laser writing (DLW). Functional DNA arrays were written by direct laser writing (DLW) in variable patterns, which were further encoded with fluorophores and proteins through DNA directed immobilization. This mild and efficient light‐driven platform technology holds promise for the fabrication of complex bioarrays with sub‐micron resolution.