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Development of a Modular Ratiometric Fluorescent Probe for the Detection of Extracellular Superoxide
Author(s) -
Andina Diana,
Brambilla Davide,
Munzinger Noah,
Frei Jasmine,
Zivko Cristina,
Leroux JeanChristophe,
Luciani Paola
Publication year - 2017
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201700563
Subject(s) - extracellular , fluorescence , chemistry , analyte , nucleic acid , linker , superoxide , peptide nucleic acid , endogeny , cell , biophysics , biochemistry , microbiology and biotechnology , biology , enzyme , chromatography , computer science , physics , quantum mechanics , operating system
Extracellular detection of endogeneous analytes (e.g., superoxide) can provide important insights into mechanisms of homeostasis and diseases, such as tumorigenesis. A ratiometric probe with a fluorescent reference and an analyte‐specific switch‐on dye was developed. Detection of ROS in the extracellular milieu was ensured by connecting the two fluorophores with a modular peptide‐nucleic‐acid‐based linker. The ROS‐sensing ability was assessed and validated in cell‐free assays and in cell culture.