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A Fluorescent‐Labeled Phosphono Bisbenzguanidine As an Activity‐Based Probe for Matriptase
Author(s) -
Häußler Daniela,
SchulzFincke AnnaChristina,
Beckmann AnnaMadeleine,
Keils Aline,
Gilberg Erik,
Mangold Martin,
Bajorath Jürgen,
Stirnberg Marit,
Steinmetzer Torsten,
Gütschow Michael
Publication year - 2017
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201700319
Subject(s) - fluorophore , proteases , fluorescence , chemistry , linker , covalent bond , serine , enzyme , coumarin , phosphonate , transmembrane protein , biochemistry , receptor , organic chemistry , physics , quantum mechanics , computer science , operating system
Activity‐based probes are compounds that exclusively form covalent bonds with active enzymes. They can be utilized to profile enzyme activities in vivo, to identify target enzymes and to characterize their function. The design of a new activity‐based probe for matriptase, a member of the type II transmembrane serine proteases, is based on linker‐connected bis‐benzguanidines. An amino acid, introduced as linker, bears the coumarin fluorophore. Moreover, an incorporated phosphonate allows for a covalent interaction with the active‐site serine. The resulting irreversible mode of action was demonstrated, leading to enzyme inactivation and, simultaneously, to a fluorescence labeling of matriptase. The ten‐step synthetic approach to a coumarin‐labeled bis‐benzguanidine and its evaluation as activity‐based probe for matriptase based on in‐gel fluorescence and fluorescence HPLC is reported. HPLC fluorescence detection as a new application for activity‐based probes for proteases is demonstrated herein for the first time.