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Dinickel–Salphen Complexes as Binders of Human Telomeric Dimeric G‐Quadruplexes
Author(s) -
Zhou ChunQiong,
Liao TingCong,
Li ZiQi,
GonzalezGarcia Jorge,
Reynolds Matthew,
Zou Min,
Vilar Ramon
Publication year - 2017
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201700276
Subject(s) - linker , chemistry , monomer , selectivity , dna , g quadruplex , titration , nuclear magnetic resonance spectroscopy , fluorescence spectroscopy , fluorescence , metal , spectroscopy , crystallography , stereochemistry , combinatorial chemistry , biochemistry , inorganic chemistry , organic chemistry , polymer , physics , quantum mechanics , computer science , catalysis , operating system
Three new polyether‐tethered dinickel–salphen complexes ( 2 a – c ) have been synthesized and fully characterized by NMR spectroscopy, mass spectrometry, and elemental analyses. The binding affinity and selectivity of these complexes and of the parent mono‐nickel complex ( 1 ) towards dimeric quadruplex DNA have been determined by UV/Vis titrations, fluorescence spectroscopy, CD spectroscopy, and electrophoresis. These studies have shown that the dinickel–salphen complex with the longest polyether linker ( 2 c ) has higher binding affinity and selectivity towards dimeric quadruplexes (over monomeric quadruplexes) than the dinickel–salphen complexes with the shorter polyether linkers ( 2 a and 2 b ). Complex 2 c also has higher selectivity towards human telomeric dimeric quadruplexes with one TTA linker than the monometallic complex 1 . Based on the spectroscopic data, a possible binding mode between complex 2 c and the dimeric G‐quadruplex DNA under study is proposed.