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Selective Luminescent Labeling of DNA and RNA Quadruplexes by π‐Extended Ruthenium Light‐Up Probes
Author(s) -
Saadallah Dounia,
Bellakhal Mehdi,
Amor Souheila,
Lefebvre JeanFrançois,
ChavarotKerlidou Murielle,
Baussanne Isabelle,
Moucheron Cécile,
Demeunynck Martine,
Monchaud David
Publication year - 2017
Publication title -
chemistry – a european journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.687
H-Index - 242
eISSN - 1521-3765
pISSN - 0947-6539
DOI - 10.1002/chem.201605948
Subject(s) - luminophore , dna , chemistry , luminescence , acridine , ruthenium , intramolecular force , photochemistry , rna , combinatorial chemistry , stereochemistry , biochemistry , materials science , organic chemistry , gene , optoelectronics , catalysis
A series of Ru II complexes exhibiting π‐extended, acridine‐based ancillary chelating heterocycles display high affinity and selectivity for DNA and RNA quadruplexes. The most promising candidates ( 3 , 4 ) possess remarkable light‐up luminophore properties (up to 330‐fold luminescence enhancement upon interaction with quadruplexes), enabling them to discriminate quadruplexes from genomic DNA owing to a photochemical mechanism involving DNA protection against non‐radiative decay (DAND), thus deviating from the other complexes of this series of ligands that exhibit an excited‐state intramolecular proton transfer (ESIPT) that quenches their luminescence. The in vitro and preliminary in cellulo results shown here confirm the interest of this new family of fluorophores as invaluable molecular tools to detect G‐quadruplexes in cells.